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Your Position: Home > Biochemical Assay Kits > Antioxidant Series > Micro Total Mercapto Assay Kit
Micro Total Mercapto Assay Kit

Storage:2-8℃

  BC1375Manual BC1375Manual
Micro Total Mercapto Assay Kit
Cat.No：
BC1375
Brand：
Solarbio
SKU Tongzhou Beijing Haidian Beijing Wuhan Guangzhou
BC1375-100T/48S Inquiry Inquiry Inquiry Inquiry
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Product Information Related Products Related Paper COA Technical Q&A

Determination of Significance：

The sulfhydryl mainly includes glutathione sulfhydryl group and protein sulfhydryl group in vivo. The former can not only repair the oxidative damage protein, but also participate in scavenging the reactive oxygen species. The latter plays an important role in maintaining the protein conformation. The content of protein sulfhydryl can be determined indirectly by measuring the content of total sulfhydryl and GSH.

Measurement Principle：

Sulfhydryl react with 5,5’-dithio-bis-nitrobenzoic acid (DTNB) to form yellow compound which has max absorbance peak at 412 nm.

Self Provided：

Spectrophotometer/microplate reader, micro glass cuvette/96 well flat-bottom plate, balance, centrifuge, constant temperature water bath, motor/homogenizer, and distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：
These protocols are for reference only. Solarbio does not independently validate these methods.

Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Experimental Images
Standard Curve for Total Mercapto(-SH) Content Assay Kit
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