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Protopectin Content Assay Kit

BC3685-241212Manual

Cat.No：BC3685 Solarbio

Detection Method：Spectrophotometer/Microplate Reader

Storage：Store at 2-8℃，6 months

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Product Information

Reagents Required But Not Provided	Please check the manual first, there are some reagents you should prepare by yourself.
Name	Protopectin Content Assay Kit
Detection Method	Spectrophotometer/Microplate Reader
Storage	Store at 2-8℃，6 months
Unit	Kit
Specification	100T/48S

Determination of Significance：

Pectin is one of the main components of plant cell wall, which is divided into water-soluble pectin and insoluble pectin. Insoluble pectin is protopectin. Raw pectin is insoluble in water, but it can be decomposed into water-soluble pectin by adding water under the action of acid, alkali, salt and other chemical reagents and enzymes. It is widely used in food, textile, printing and dyeing, tobacco, metallurgy and other fields.

Measurement Principle：

The protopectin is hydrolyzed to soluble pectin under alkaline condition, and further converted to galacturonic acid. The product condensed with carbazole in strong acid to form purplish red compound, with characteristic absorption peak at 530 nm.

Self Provided：

Spectrophotometer/microplate reader, desktop centrifuge, water bath, micro glass cuvette/96 well flat-bottom plate, adjustable pipette, mortar/homogenizer, acetone, concentrated sulfuric acid (H2SO4), anhydrous ethanol and distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.

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