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My Cart> Biochemical Assay Kits > Energy Metabolism Series > Mitochondrial Respiratory Chain Series > Micro Mitochondrial Respiratory Chain ComplexⅤActivity Assay Kit Detection Method：Spectrophotometer/Microplate Reader
Storage：Store at -20℃，6 months
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| Name | Micro Mitochondrial Respiratory Chain ComplexⅤActivity Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at -20℃，6 months |
| Unit | Kit |
| Specification | 100T/48S |
Determination of Significance：
Mitochondrial Respiratory Chain Complex V also known as F1F0-ATP synthase, widely exists in mitochondria of animals, plants, microorganisms and cultured cells, and consists of two subunits F1 and F0.The enzyme catalyzes the synthesis of ATP using the proton electrochemical gradient generated by respiratory chain, and also hydrolyzes ATP in a reversible process. In addition, complex V is also present in chloroplasts, heterotrophs and photosynthetic bacteria. Complex V is the key enzyme for ATP synthesis from mitochondrial oxidative phosphorylation and chloroplast photophosphorylation.
Measurement Principle：
Complex V hydrolyzes ATP to produce ADP and Pi. Molybdenum blue can react with inorganic phosphorus, the reaction product can be detected by colorimetric assay at 660 nm and determine the Complex V activity indirectly.
Self Provided：
Spectrophotometer/Microplate Reader, Water Bath/Constant Temperature Incubator, Desk Centrifuge, Adjustable Transferpettor, Micro Glass Cuvette/ 96 Well Flat-Bottom Plate, Mortar/Homogenizer/Cell Ultrasonic Crusher, Ice and Distilled Water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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