Determination of Significance:
GLS (EC3.5.1.2) is mainly found in higher animals, some bacteria and plant roots, catalyze the hydrolysis of glutamine into glutamic acid and ammonia, which plays an important role in the regulation of nitrogen metabolism, especially the regulation of free ammonia and urea metabolism.
Measurement Principle:
The kit uses the indophenol blue colorimetric method to determine ammonia produced by glutamine of GLS-catalyzed to indicate activity.
Self Provided:
Spectrophotometer/microplate reader, adjustable pipette, balance, mortar/homogenizer, centrifuge, micro glass cuvette/96 well flat-bottom plate, ice and distilled water.
Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.