English
中文
Determination of Significance：
DH (EC 1.4.1.2) is widely distributed in plants. GDH and glutamate synthetase (GOGAT) are involved in the synthesis of glutamate. It plays an important role in the assimilation of ammonia and the metabolism of organic nitrogen compounds.
Measurement Principle：
GDH catalyzes the NH4+, α-ketoglutaric acid and NADH to form glutamic acid and NAD+, which cause a decrease in absorbance at 340 nm. The GDH activity is calculated by measuring the rate of decrease in absorbance at 340 nm.
Self Provided：
Spectrophotometer/Microplate reader, desk centrifuge, adjustable pipette, water bath, micro quartz cuvette/ 96-well flat-bottom plate (UV), mortar/homogenizer, ice and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
