Micro NO Content Assay Kit
Cat.No：BC1475 Solarbio
Detection Method：Spectrophotometer/Microplate Reader
Storage：Store at -20℃，6 months
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> Biochemical Assay Kits > Other Biochemical Series > Signal Series > Micro NO Content Assay Kit
Product Information
| Name | Micro NO Content Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at -20℃，6 months |
| Unit | Kit |
| Specification | 100T/96S |
Determination of Significance：
Nitric Oxide (NO) is a highly unstable biological free radical with small molecule, simple structure，NO is gas at room temperature, slightly soluble in water and fat-soluble, which can diffuse through biofilms quickly. As a new type of biological messenger molecule, it plays a role in transmitting signals between cells and within cells. It is widely distributed in various tissues, especially neural tissues. It also plays a very important role in the nervous, circulatory, respiratory, digestive, and urogenital systems of the body.
Measurement Principle：
NO is easily oxidized to form NO2- and NO3- in the body or in aqueous solution. This method uses nitrate reductase to reduce NO3- to NO2- specifically. Under acidic conditions, NO2- and diazonium sulfonamide produce diazo compounds. The compounds could further couple with naphthyl vinyl diamine, the product has a characteristic absorption peak at 550 nm, and its absorbance value can be measured to calculate the NO content.
Self Provided：
Spectrophotometer/ microplate reader, low temperature centrifuge, water bath/constant temperature incubator, adjustable pipette, micro glass cuvette/96-well plate, mortar/homogenizer, ice and distilled water, EP tube.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Experimental Images
Standard Curve for Nitric Oxide(NO) Content Assay Kit
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