Determination of Significance:
Nitrite becomes more stable when bonding with myoglobin in food. It can be used as color preserving reagent to maintain good appearance of meat products and prevent producing clostridium botulinum toxin. It can improve the safety of meat products. It also may cause cancer of digestive system if body intakes too much for long time.
Measurement Principle:
In acidic condition, nitrite reacts with P-aminobenzene sulfonic acid and form diazo-compound, which can react with N-1-naphthalene ethylenediamine to form purple-red azoic-compound. It has a characteristic absorption peak at 540 nm.
Self Provided:
Spectrophotometer/microplate reader, micro glass cuvette/96 well flat-bottom plate, homogenizer/mortar, balance, water bath,distilled water.
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