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Determination of Significance：
Nitrate is one of the nitrogen - containing substances absorbed by plants. Nitrate is reduced in roots , branches or leaves, depending on plant type and environmental conditions. Detecting nitrate nitrogen content in plants is significant to understand the nitrogen metabolism mechanism.
Measurement Principle：
NO3- can react with salicylic acid to form nitrosalicylic acid under the condition of concentrated acid, which shows yellow under the condition of pH>12. Within a certain range, the color depth is proportional to the content.
Self Provided：
Spectrophotometer/microplate reader, micro glass cuvette/96 well flat-bottom plate,?water bath, desk centrifuge, mortar/homogenizer, ice and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
