Determination of Significance:
Soil ammonium nitrogen is an important component of soil available nitrogen, which can be directly absorbed and utilized by plants. Ammonium nitrogen index plays an important role in agricultural production.
Measurement Principle:
In the strong alkaline medium, the ammonium nitrogen in the soil interacts with hypochlorite and phenol to form indophenol blue dye, which has a characteristic absorption peak at 630 nm, and the absorption value is directly proportional to the ammonium nitrogen content.
Self Provided:
Spectrophotometer/microplate reader, scale, centrifuge, micro glass cuvette/96 well flat-bottom plate, oscillator, water bath.
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