Alkaline Phosphatase(AKP/ALP) Activity Assay Kit
Cat.No：BC2145 Solarbio
Detection Method：Spectrophotometer/Microplate Reader
Storage：Store at 2-8℃，6 months
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Angiogenesis
HIF
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> Biochemical Assay Kits > Protein ＆ Enzyme Series > Esterase Series > Alkaline Phosphatase(AKP/ALP) Activity Assay Kit
Product Information
| Name | Alkaline Phosphatase(AKP/ALP) Activity Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at 2-8℃，6 months |
| Unit | Kit |
| Specification | 100T/48S |
Determination of Significance：
AKP/ALP is a zinc-containing glycoprotease, which hydrolysis various natural and synthetic phospholipid monoester compounds in alkaline condition. AKP / ALP are widely distributed in human organs, mainly in liver.
Measurement Principle：
In alkaline condition, AKP/ALP catalyzes hydrolysis disodium phenyl phosphate to phenol, and the phenol reacts with 4-Aminoantipyrine and potassium ferricyanide to form red quinone derivative, which can be detect absorbance at 510 nm. AKP/ALP activity can be calculated by measuring the absorbance increase rate at 510 nm.
Self Provided：
Spectrophotometer/Microplate Reader, Micro Glass Cuvette/96 Well Flat-Bottom Plate, Transferpettor, Desk Centrifuge, Ice and Distilled Water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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