Determination of Significance:
Manganese peroxidase (EC1.11.1.13) is a microbial lignin decomposing enzyme widely existing in bacteria and fungi. It plays a key role in the microbial lignin decomposing system. It can effectively degrade lignin and refractory compounds in wastewater and soil. It is widely used in industrial fields such as biological pulping, biological bleaching and biodegradation of pollutants.
Measurement Principle:
Manganese peroxidase oxidizes guaiacol to tetra-o-methoxyl phenol in the presence of Mn2+, with a characteristic absorption peak at 465 nm.
Self Provided:
Spectrophotometer/Microplate Reader, Micro quartz cuvette/96 well plate, Low temperature centrifuge, Water bath/Constant temperature incubatorbalance, Pipette, Cell Sonicator, Mortar/ homogenizer, Ice and distilled water.
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