Determination of Significance:
Laccase (EC1.10.3.2) is a polyphenol oxidase containing copper. It belongs to the ceruloplasmic oxidase family. Laccase is a kind of environmental protection enzyme which exists in mushroom, fungus and plant. Its unique catalytic properties are widely used in biological detection.
Measurement Principle:
Laccase can decompose substrate ABTS to produce ABTS free radicals. Its absorption coefficient at 420nm is much higher than that of ABTS. Laccase activity can be calculated by measuring the increasing rate of ABTS radicals.
Self Provided:
Spectrophotometer/microplate reader, balance, low temperature desk centrifuge, transferpettor, oscillating instrument, micro glass cuvette/96 well flat-bottom plate, mortar/homogenizer, water bath, ice and distilled water.
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