Determination of Significance:
Serum iron is the iron bound with transferrin in blood, which is often used to distinguish non-iron deficiency anemia and iron-deficiency anemia
Measurement Principle:
Fe3+ is reduced by sodium sulfite to Fe2+, which reacts with 2,2-dipyridine-bipyridine, have an absorption peak at 520 nm. According measure absorbance at 520 nm can reflect serum iron concentration.
Self Provided:
Spectrophotometer/Microplate Reader, Centrifuge, Micro Glass Cuvette/96 Well Flat-bottom Plate, Glacial Acetic Acid, Adjusted Transferpettor, Chloroform and Distilled Water.
Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark:
These protocols are for reference only. Solarbio does not independently validate these
methods.
Note:
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