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Determination of Significance：
Chitin is the second largest class of biopolymers in nature, which is second only to cellulose. Due to its slow decomposition and large accumulation, it is easy to cause serious environmental pollution. Chitinase is an important enzyme affecting nitrogen mineralization in soil, and its decomposition of chitin controls the key step of nitrogen cycle.
Measurement Principle：
Chitinase hydrolyzes chitin to produce N-acetylglucosamine. The intermediate compound produced by the reaction of N-acetylglucosamine and alkali can further react with p-Dimethylaminobenzaldehyde to produce a chromogenic substance. The chromogenic substance has a characteristic absorption peak at 585 nm. The increasing rate of absorbance reflects the activity of chitinase.
Self Provided：
Spectrophotometer/microplate reader, Low tempareture centrifuge, transferpettor, oscillator, micro glass cuvette/96 well flat-bottom plate, mortar, 30-50 mesh sieve (or smaller), distilled water and toluene, water bath or constant temperature incubator.
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