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Determination of Significance：
Soil manganese peroxidase (EC1.11.1.13) is a kind of peroxidase containing met Hb. It widely exists in white rot basidiomycetes, belongs to the lignin degrading enzyme system, and is the key enzyme for initial degradation of lignin. It can effectively degrade lignin and chlorides, azides, DTTs, polycyclic hydrocarbons, etc. that are difficult to degrade in wastewater and soil, It has more research and application in agricultural waste treatment, biodegradation, biobleaching, dye decolorization and other fields.
Measurement Principle：
Manganese peroxidase oxidizes guaiacol to tetra-o-methoxycophenol in the presence of Mn2+, with a characteristic absorption peak at 465 nm.
Self Provided：
Spectrophotometer/Microplate Reader, Centrifuge, Water-bath/Constant Temperature Incubator, Transferpettor, Micro Glass Cuvette/96 Well Flat-bottom Plate, Oscillator, Toluene, 30-50 Mesh Sieve and Distilled Water.
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