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Determination of Significance：
Total cholesterol (TC) is the sum of cholesterol contained in all lipoproteins, including free cholesterol and cholesteryl esters.
Measurement Principle：
The enzyme esterase catalyzes the hydrolysis of cholesteryl esters to produce free cholesterol (FC) and free fatty acids (FFA), thus converting cholesteryl esters to FC; furthermore, cholesteryl oxidase catalyzes the oxidation of FC to produce 4-Cholestenone and H2O2; finally, peroxidase catalyzes the oxidation of 4-aminoantipyrine and phenol by H2O2 to produce red quinones, which have a characteristic absorption peak at 500 nm. The color shade is proportional to the TC content.
Self Provided：
Spectrophotometer/microplate reader, balance, low temperature table centrifuge, water-bath, micro glass cuvette/96 well flat-bottom plate, pipette, EP tube, distilled water, isopropyl alcohol.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
2023-08-10 11:44:41
请问用组织提取方法提取肝脏中的总胆固醇后，再用试剂盒进行检测时出现大量白色混浊物影响吸光度如何处理呢2023-05-25 08:42:57
Administrator您好，需要使用本试剂盒中提取液，按照说明书中组织样本处理方式处理样本。