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Your Position: Home > Biochemical Assay Kits > Other Series > Isocitrate Lyase(ICL) Activity Assay Kit
Isocitrate Lyase(ICL) Activity Assay Kit

Storage:-20℃

  BC2035Manual
Isocitrate Lyase(ICL) Activity Assay Kit
Cat.No：
BC2035
Brand：
Solarbio
SKU Tongzhou Beijing Haidian Beijing Wuhan Guangzhou
BC2035-100T/96S Inquiry Inquiry Inquiry Inquiry
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Product Information Related Products Related Paper COA Technical Q&A

Determination of Significance：

ICL (ec4.1.3.1) mainly exists in plants and microorganisms. During the germination process of oil seeds, fat is converted into carbohydrate through glyoxylate cycle and other processes. ICL is one of the key enzymes in glyoxylate cycle.

Measurement Principle：

The degradation of isocitric acid to glyoxylic acid and succinic acid is catalyzed by ICL. Glyoxylic acid and NADH generate ethanol and NAD under the action of LDH. NADH has a characteristic absorption peak at 340nm. Monitoring the reduction rate of 340nm absorbance can indirectly reflect the activity of ICL.

Self Provided：

Spectrophotometer/ Microplate reader, water bath, desk centrifuge, adjustable transferpettor, micro quartz cuvette/ 96 well flat-bottom plate, mortar / homogenizer, ice and distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：
These protocols are for reference only. Solarbio does not independently validate these methods.

Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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