6-Phosphogluconate ehydrogenase(6PGDH)Activity Assay Kit
Cat.No:BC2105 Solarbio
Detection Method:Spectrophotometer/Microplate Reader
Storage:Store at -20℃,6 months
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6-Phosphogluconate ehydrogenase(6PGDH)Activity Assay KitDetection Method:Spectrophotometer/Microplate Reader
Storage:Store at -20℃,6 months
Qty:
Size:
Name | 6-Phosphogluconate ehydrogenase(6PGDH)Activity Assay Kit |
Detection Method | Spectrophotometer/Microplate Reader |
Storage | Store at -20℃,6 months |
Recommend | If 96-well plate is used for determination, 96-well UV plate should be used, recommended item numberYA0602 |
Unit | Kit |
Specification | 100T/96S |
Determination of Significance:
The 6-phosphate glucose dehydrogenase (6-PGDH) and 6-phosphogluconate dehydrogenase (6-PGDH) in the pentose phosphate pathway catalyze the synthesis of NADPH in turn, which is closely related to energy balance, growth rate and cell viability. In addition, 6-PGDH plays an important role in stress physiology.
Measurement Principle:
6-PGDH catalyzes the production of NADPH by 6-phosphogluconic acid and NADP+. NADPH has a characteristic absorption peak at 340 nm, while NADP+ does not. In this kit, the activity of 6-PGDH is determined by the increase rate of NADPH at 340 nm.
Self Provided:
Ultraviolet spectrophotometer/microplate reader, desk centrifuge, pipette, water bath, micro quartz cuvette/96 well UV plate, mortar/homogenizer, ice, distilled water.
Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark:These protocols are for reference only. Solarbio does not independently validate these methods.
Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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Potential xylose transporters regulated by CreA improved lipid yield and furfural tolerance in oleaginous yeast Saitozyma podzolica zwy-2-3
Click to check >>Author:Yulu Ran, Qingzhuoma Yang, Jie Zeng, Fazhi Li, Yu Cao, Qingrui Xu, Dairong Qiao, Hui Xu, Yi Cao
IF:11.4000
Publish_to:BIORESOURCE TECHNOLOGY
PMID:37390935