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Determination of Significance：
In acid condition, ACP catalyze phosphomonoester to inorganic phosphate, which is found in lysozyme of macrophages. ACP can be used in auxiliary diagnosis of prostate cancer.
Measurement Principle：
In acid condition, ACP catalyzes hydrolysis disodium phenyl phosphate to phenol, and the phenol reacts with 4-Aminoantipyrine and potassium ferricyanide to form red quinone derivative, which can be detect absorbance at 510 nm. ACP activity can be calculated by measuring the absorbance increase rate at 510 nm.
Self Provided：
Spectrophotometer/Microplate Reader, Micro Glass Cuvette/96 Well Flat-Bottom Plate, Transferpettor, Desk Centrifuge, Mortar/Homogenizer, Ice and Distilled Water.
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