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Determination of Significance：
CA is a common organic acid in organism and an important food flavor substance. In addition, CA is the product of the first step of the tricarboxylic acid cycle.
Measurement Principle：
Under acid condition, Cr6+ is reduced to Cr3+ by citric acid, which have a characteristic absorption peak at 545 nm. The content of citric acid in the sample can be calculated by measuring the increase of the absorption value at 545 nm.
Self Provided：
Spectrophotometer/plate reader, cryogenic centrifuge, water bath, adjustable pipette, micro glass cuvettes/96-well plate, mortar/homogenizer, ice and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：
These protocols are for reference only. Solarbio does not independently validate these
methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Experimental Images
Standard Curve for Citric Acid(CA) Content Assay Kit
