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Determination of Significance：
L (+)-Lactic acid is an important intermediate product in biological metabolism. It is closely related to glucose metabolism, lipid metabolism, protein metabolism and intracellular energy metabolism. Lactic content is an important indicator for assessing carbohydrate metabolism and aerobic metabolism.
Measurement Principle：
L (+)-Lactic produces pyruvic acid?under the action of lactate dehydrogenase, and NAD+ is reduced to produce NADH and H+. H+ is transferred to PMS to produces PMSH2 and PMSH2 reduce MTT to form purple substance, and has a characteristic absorption peak at 570 nm.
Self Provided：
Scale, mortar/homogenizer, centrifuge, spectrophotometer/microplate reader, micro glass cuvette/ 96 well flat-bottom plate, constant temperature water bath, ethanol, distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
你好，请问能测组织样品吗2023-07-20 20:24:23
Administrator您好，可以检测组织、细胞。血清等样本
微量法也需要分光光度？2022-08-22 14:38:26
Administrator微量法可以用微量玻璃比色皿或者96孔板去测，如果用微量比色皿也是需要分光光度计的
您好！请问测量用高糖和1640培养基培养的细胞，其乳酸化水平可以使用这款试剂盒吗？2022-08-09 11:07:48
Administrator您好，试剂盒没有对样本来源等要求，如果测细胞内的离心收集细胞沉淀，如果测培养的上清液按血清这类液体样本处理方式来做，试剂盒技术指标有检出限和线性范围要求，能否检测到取决于浓度了
酶标仪检测和分光光度计检测有什么区别吗
2022-08-02 15:42:21
Administrator您好，试剂盒开发有微量法和分光光度法两种不同大小反应体系的，原理是相同的，检测限以及测定方式是有区别的，您可以根据样本以及实验室现有的仪器设备来选择
试剂盒保存时间多久？2022-07-19 20:02:40
Administrator试剂盒未拆封情况下，生产日期开始有效期为六个月