Lactic Acid(LA) Content Assay Kit
Cat.No：BC2235 Solarbio
Detection Method：Spectrophotometer/Microplate Reader
Storage：Store at -20℃，6 months
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> Biochemical Assay Kits > Carbohydrate Metabolism Series > Glycolysis Series > Lactic Acid(LA) Content Assay Kit
Product Information
| Reagents Required But Not Provided | Please check the manual first, there are some reagents you should prepare by yourself. |
| Name | Lactic Acid(LA) Content Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at -20℃，6 months |
| Unit | Kit |
| Specification | 100T/48S |
Determination of Significance：
L (+)-Lactic acid is an important intermediate product in biological metabolism. It is closely related to glucose metabolism, lipid metabolism, protein metabolism and intracellular energy metabolism. Lactic content is an important indicator for assessing carbohydrate metabolism and aerobic metabolism.
Measurement Principle：
L (+)-Lactic produces pyruvic acid?under the action of lactate dehydrogenase, and NAD+ is reduced to produce NADH and H+. H+ is transferred to PMS to produces PMSH2 and PMSH2 reduce MTT to form purple substance, and has a characteristic absorption peak at 570 nm.
Self Provided：
Scale, mortar/homogenizer, centrifuge, spectrophotometer/microplate reader, micro glass cuvette/ 96 well flat-bottom plate, constant temperature water bath, ethanol, distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Experimental Images
Standard Curve for Lactic Acid(LA) Content Assay Kit
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