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Measurement significance:
Fructose-1,6-Diphosphate (FDP) is an important intermediate product in the process of glycolysis.It has a regulatory effect on a variety of enzymes,and can improve cell energy metabolism,increase energy utilization anti-arrhythmia and anti-tissue peroxidation.It is widely used in clinical medicine.
Determination method:
Aldolase catalyzes fructose 1,6-diphosphate to produce glyceraldehyde-3-phosphate and dihydroxyacetone phosphate.Under the action of triose phosphate isomerase and α-glycerophosphate dehydrogenase,NADH and dihydroxyacetone phosphate are catalyzed to produce NAD+ and α-glycerophosphate, causing a decrease in absorbance at 340 nm.The content of fructose 1,6-diphosphate can be calculated by measuring the change of NADH.
Instruments and supplies to be provided:
Spectrophotometer/Microplate Reader,balance,low temperature desktop centrifuge,water bath/thermostatic incubator,1mL quartz cuvette,adjustable pipette,mortar/homogenizer / cell ultrasonic disruptor,vortex oscillator,ice,distilled water and EP tube.
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