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Your Position: Home > Biochemical Assay Kits > Glycolysis Series > Phosphoglycerate Kinase(PGK) Activity Assay Kit
Phosphoglycerate Kinase(PGK) Activity Assay Kit

Storage:-20℃

  BC2255Manual
Phosphoglycerate Kinase(PGK) Activity Assay Kit
Cat.No：
BC2255
Brand：
Solarbio
SKU Tongzhou Beijing Haidian Beijing Wuhan Guangzhou
BC2255-100T/96S Inquiry Inquiry Inquiry Inquiry
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Product Information Related Products Related Paper COA Technical Q&A

Determination of Significance：

PGK is the key enzyme of glycolysis. It's also a key enzyme for organisms to survive. It widely exists in animals, plants and microorganisms. It has many biological functions, such as affecting DNA replication and repair, stimulating RNA synthesis of virus, and is widely used in drug target design.

Measurement Principle：

PGK catalyzes the production of 1,3-diphosphoglyceride and ADP from 3-phosphoglycerate and ATP. 1,3-diphosphoglyceride produces 3-phosphoglyceraldehyde, NAD and phosphoric acid under the action of 3-phosphoglyceraldehyde dehydrogenase and NADH. The absorbance decreased at 340nm. It reflects the activity of 3-phosphoglycerate kinase.

Self Provided：

Ultraviolet spectrophotometer/microplate reader, desk centrifuge, water-bath, transferpettor, micro quartz cuvette/96 well UV plate, mortar/homogenizer, ice and distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：
These protocols are for reference only. Solarbio does not independently validate these methods.

Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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