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Determination of Significance：
PGK is the key enzyme of glycolysis. It's also a key enzyme for organisms to survive. It widely exists in animals, plants and microorganisms. It has many biological functions, such as affecting DNA replication and repair, stimulating RNA synthesis of virus, and is widely used in drug target design.
Measurement Principle：
PGK catalyzes the production of 1,3-diphosphoglyceride and ADP from 3-phosphoglycerate and ATP. 1,3-diphosphoglyceride produces 3-phosphoglyceraldehyde, NAD and phosphoric acid under the action of 3-phosphoglyceraldehyde dehydrogenase and NADH. The absorbance decreased at 340nm. It reflects the activity of 3-phosphoglycerate kinase.
Self Provided：
Ultraviolet spectrophotometer/microplate reader, desk centrifuge, water-bath, transferpettor, micro quartz cuvette/96 well UV plate, mortar/homogenizer, ice and distilled water.
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