Phosphoglycerate Kinase(PGK) Activity Assay Kit
Cat.No：BC2255 Solarbio
Detection Method：Spectrophotometer/Microplate Reader
Storage：Store at -20℃，6 months
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Endocrinology & Hormones
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HIF
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> Biochemical Assay Kits > Carbohydrate Metabolism Series > Glycolysis Series > Phosphoglycerate Kinase(PGK) Activity Assay Kit
Product Information
| Name | Phosphoglycerate Kinase(PGK) Activity Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at -20℃，6 months |
| Recommend | If 96-well plate is used for determination, 96-well UV plate should be used, recommended item numberYA0602 |
| Unit | Kit |
| Specification | 100T/96S |
Determination of Significance：
PGK is the key enzyme of glycolysis. It's also a key enzyme for organisms to survive. It widely exists in animals, plants and microorganisms. It has many biological functions, such as affecting DNA replication and repair, stimulating RNA synthesis of virus, and is widely used in drug target design.
Measurement Principle：
PGK catalyzes the production of 1,3-diphosphoglyceride and ADP from 3-phosphoglycerate and ATP. 1,3-diphosphoglyceride produces 3-phosphoglyceraldehyde, NAD and phosphoric acid under the action of 3-phosphoglyceraldehyde dehydrogenase and NADH. The absorbance decreased at 340nm. It reflects the activity of 3-phosphoglycerate kinase.
Self Provided：
Ultraviolet spectrophotometer/microplate reader, desk centrifuge, water-bath, transferpettor, micro quartz cuvette/96 well UV plate, mortar/homogenizer, ice and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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Related Paper
Validation of protein biological markers of lamb meat quality characteristics based on the different muscle types
Click to check >>Author：Caiyan Huang, Dequan Zhang, Zhenyu Wang, Yingxin Zhao, Christophe Blecker, Shaobo Li, Xiaochun Zheng, Li Chen
IF：8.8000
Publish_to：FOOD CHEMISTRY
PMID：37392625
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