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Triose Phosphate Isomerase (TPI) Activity Assay Kit

BC2265-241212Manual

Cat.No：BC2265 Solarbio

Detection Method：Spectrophotometer/Microplate Reader

Storage：Store at -20℃，6 months

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Product Information

Name	Triose Phosphate Isomerase (TPI) Activity Assay Kit
Detection Method	Spectrophotometer/Microplate Reader
Storage	Store at -20℃，6 months
Recommend	If 96-well plate is used for determination, 96-well UV plate should be used, recommended item numberYA0602
Unit	Kit
Specification	100T/96S

Determination of Significance：

Triose phosphate isomerase in plant chloroplasts is an important enzyme involved in the calvin cycle in photosynthesis. Triose phosphate isomerase acts on the conversion between glyceraldehyde phosphate and dihydroxyacetone phosphate. Dihydroxyacetone phosphate can quickly penetrate the chloroplast envelope into the cytoplasm, where it is gradually converted into sucrose.

Measurement Principle：

Triose phosphate isomerase converts dihydroxyacetone phosphate to glyceraldehyde 3-phosphate. Glyceraldehyde 3-phosphate and NAD generate 3-phosphoglycerate and NADH under the action of glyceraldehyde 3-phosphate dehydrogenase. The change in absorbance at 340nm reflects the activity of triose phosphate isomerase.

Self Provided：

Ultraviolet spectrophotometer/Microplate reader, balance, water-bath, centrifuge, transferpettor, micro quartz cuvette/96 well UV plate, mortar/homogenizer, ice and distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.

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