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Determination of Significance：
Fructose 1,6 bisphosphate aldolase (FBA) (EC4.1.2.13) is an important part of the Calvin cycle in glycolysis, gluconeogenesis, pentose phosphate pathway, and photosynthesis Enzymes, which catalyze the reversible cleavage of fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate, are widely present in plants, animals and microorganisms, and exhibit different responses under various stress conditions.
Measurement Principle：
FBA catalyzes the production of fructose 1,6-diphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate, and catalyzes NADH and triphosphate under the action of triose phosphate isomerase and α-phosphate glycerol dehydrogenase. Dihydroxyacetone phosphate produces NAD and α-phosphoglycerin. The change in absorbance at 340nm can reflect the level of fructose 1,6-bisphosphate aldolase activity.
Self Provided：
Ultraviolet spectrophotometer/microplate reader, desk centrifuge, adjustable transferpettor, micro quartz cuvette/ 96 well UV plate, mortar/homogenizer, vortex oscillator, ultrasonic crusher, ice and distilled water.
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