Determination of Significance:
NP catalyze the hydrolysis of protein under certain temperature and neutral PH conditions. It has the characteristics of safety, non-toxicity, strong hydrolysis ability and wide range of action. So NP is often used in the production of food, feed, cosmetics and nutritional health products.
Measurement Principle:
In neutral conditions, NP can catalyze the hydrolysis of casein to produce tyrosine. In alkaline condition, tyrosine reduces phosphomolybdic acid compounds to tungsten blue, which has a characteristic absorption peak at 680 nm.
Self Provided:
Mortar/homogenizer, desk centrifuge, spectrophotometer/microplate reader, micro glass cuvette/96 well flat-bottom plate, water bath, magnetic stirrer, transferpettor, 1.5 mL centrifuge tube and distilled water.
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