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Determination of Significance：
AKP is a serine protease, which catalyzes the hydrolysis of protein peptide bond in alkaline condition. In addition, the enzyme can hydrolyze ester bond and amide bond. It has the function of trans ester and trans peptide. The enzyme is one of the main industrial enzymes, which is widely used in pharmaceutical, silk, food, leather and other industries.
Measurement Principle：
In alkaline condition, AKP hydrolyzes casein to produce tyrosine. In alkaline condition, tyrosine reduced phosphomolybdic acid to tungsten blue. Tungsten blue has a characteristic absorption peak at 680 nm. The activity of AKP can be calculated by measuring the increasing rate of 680 nm absorbance.
Self Provided：
Mortar/homogenizer, desk centrifuge, spectrophotometer/microplate reader, micro glass cuvette/96 well flat-bottom plate, water bath, magnetic stirrer, transferpettor, 1.5 mL centrifuge tube and distilled water.
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