Trypsase Activity Assay Kit
Cat.No:BC2315 Solarbio
Detection Method:Spectrophotometer/Microplate Reader
Storage:Store at -20℃,6 months
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Trypsase Activity Assay KitDetection Method:Spectrophotometer/Microplate Reader
Storage:Store at -20℃,6 months
Qty:
Size:
Name | Trypsase Activity Assay Kit |
Detection Method | Spectrophotometer/Microplate Reader |
Storage | Store at -20℃,6 months |
Unit | Kit |
Specification | 100T/96S |
Determination significance:
Trypsin ( EC 3.4.4.4 ) selectively hydrolyzes the peptide chain composed of the carboxyl groups of lysine or arginine in denatured proteins and is an important digestive enzyme. In addition,trypsin is also widely used in the adjuvant treatment of local edema,,hematoma and abscess caused by empyema,hemothorax,surgical inflammation,ulcer and traumatic injury.
Determination principle:
Trypsin catalyzes the substrate BAPNA to produce p-nitroaniline ( p-NA ),which has a characteristic absorption peak at 405 nm wavelength. Since the absorbance value of p-NA is proportional to the content,the enzyme activity of trypsin can be calculated by measuring the p-NA produced per unit time.
Instruments and supplies:
Spectrophotometer/microplate reader,microglass cuvette/96-well plate,centrifuge,water bath,adjustable pipette,mortar/homogenizer,ice and distilled water.
Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark:These protocols are for reference only. Solarbio does not independently validate these methods.
Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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The duration of food withdrawal affects the intestinal structure, nutrients absorption and utilization in broiler chicken
Click to check >>Author:王友利
IF:5.3000
Publish_to:
PMID:33190300