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Determination of Significance：
Chymotrypsin, is a protein hydrolase secreted by the pancreas that rapidly breaks down denatured proteins. The function of chymotrypsin is similar to that of trypsin, but chymotrypsin has the advantages of high catabolic capacity, low toxicity and low adverse effects. Chymotrypsin is used clinically for sputum thinning, and is effective in both purulent and non-purulent sputum; chymotrypsin is also used for wound healing after trauma or surgery, such as cataract extraction.
Measurement Principle：
Chymotrypsin catalyzes the hydrolysis of BTEE and the product has characteristic absorption at 256 nm; chymotrypsin activity is calculated by measuring the rate of increase in 256 nm light absorption.
Self Provided：
Ultraviolet spectrophotometer/microplate reader, micro quartz cuvette/ 96 well UV plate, water bath, desk centrifuge, transferpettor, mortar/homogenizer, ice and distilled water.
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