Hepatic lipase(HL) Activity Assay Kit
Cat.No:BC2385 Solarbio
Detection Method:Spectrophotometer/Microplate Reader
Storage:Store at -20℃,6 months
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Fatty Acid Metabolism Series >
Hepatic lipase(HL) Activity Assay KitDetection Method:Spectrophotometer/Microplate Reader
Storage:Store at -20℃,6 months
Qty:
Size:
Reagents Required But Not Provided | Please check the manual first, there are some reagents you should prepare by yourself. |
Name | Hepatic lipase(HL) Activity Assay Kit |
Detection Method | Spectrophotometer/Microplate Reader |
Storage | Store at -20℃,6 months |
Unit | Kit |
Specification | 100T/48S |
Determination of Significance:
Hepatic lipase (HL) is a lipolytic enzyme synthesized in liver parenchymal cells. It is present on the surface of the liver sinusoidal endothelial cells and the surface of the hepatocyte microvilli in the sinusoidal space, and can hydrolyze various lipoproteins. The triglycerides (TG) and phospholipids (PL) in the medium change the size and density of various lipoprotein particles. When the HL and its activity in the plasma increasing, it can lead to low density lipoprotein (LDL) levels in the plasma, increase and accelerate the occurrence and development of atherosclerosis.
Measurement Principle:
HL hydrolyzes α-naphthyl acetate to produce α-naphthol, which can form a purple-red azo compound with fast blue B salt. It has a characteristic absorption peak at 595 nm, and its color depth is positively correlated with liver esterase activity within a certain range.
Self Provided:
Spectrophotometer/ microplate reader, water bath, balance, centrifuge, adjustable transferpettor, micro glass cuvette/ 96 well flat-bottom plate, mortar/homogenizer/ sonicator, ice and distilled water.
Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark:These protocols are for reference only. Solarbio does not independently validate these methods.
Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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