| Product Type |
Peptides |
| CAS |
190383-13-2 |
| Sequence(1LC) |
SLIGKV-NH2 |
| Sequence(3LC) |
Ser-Leu-Ile-Gly-Lys-Val-NH2 |
| Synonyms |
蛋白酶活化的受体-2，酰胺;Proteinase Activated Receptor 2,amide;Proteinase Activated Receptor 2 (1-6) amide (human); SLIGKVamide; Thrombin Receptor-Like 1 (1-6) amide (human); Coagulation Factor II Receptor-Like 1 (1-6) amide (human);PAR-2 (1-6) amide (human) |
| Molecular Formula |
C28H54N8O7 |
| Molecular Weight |
614.79 |
| Purity |
≥95% |
| Appearance |
Lyophilized powder |
| Solubility |
H2O：33.33 mg/mL;
*Ultrasonic solubilization; |
| Salt Form |
Trifluoroacetate salt |
| Source |
Synthetic |
| Storage |
Store at -20℃，2 years.(Avoid freeze/thaw cycles) |
| Category/Label |
Other Peptides |
| Background |
SLIGKV-amide, which corresponds to the tethered ligand sequence of human PAR-2 (protease activated receptor 2), can be used to investigate receptor functions. |
| In Vitro |
The PAR2-activating peptides used are: SLIGKV-OH, SLIGRL-OH, SLIGKV-NH2, SLIGRL-NH2. The synthetic agonist peptides mimicking the tethered ligand of PAR2, Ser-Leu-Ile-Gly-Lys-Val (SLIGKV-OH), Ser-Leu-Ile-Gly-Arg-Leu (SLIGRL-OH) and their amidated forms Ser-Leu-Ile-Gly-Lys-Val-amide (SLIGKV-NH2) Ser-Leu-Ile-Gly-Arg-Leu-amide (SLIGRL-NH2) have also been demonstrated being able to activate the receptor without enzymatic cleavage, therefore, have been utilised as biological tools to examine physiological functions of PAR2. Protease-Activated Receptor-2, amide is one of a four family subgroup of G-protein-coupled receptors (GPCRs), called PARs. Protease-activated receptors are distinguished from other GPCRs through their unique proteolytic mechanism of activation. For PAR2, activating proteases, such as trypsin, tryptase and coagulation factors VIIa and Xa, cleave a specific extracellular amino-terminal domain of the receptor to reveal a "tethered ligand", SLIGKV- and SLIGRL- for human and mouse/rat PAR2, respectively, which subsequently interacts with the activation domain of the receptor, initiating intracellular signaling pathways.
The protease-activated receptor-2 (PAR2) has been implicated in the pathogenesis of several inflammatory and autoimmune disorders, and is expressed in a wide variety of human tissues and cells. PAR2 belongs to a family of seven transmembrane domain receptor proteins that are activated by proteolysis. Enzymatic digestion exposes an N-terminus ligand sequence that binds intramolecularly to the activation site on the extracellular loop II, initiating a G-protein-mediated cell-signalling cascade and nuclear factor-kappa B (NF-κB)-regulated gene transcription. |
| Reference |
[1] Dulon et al. Am. J. Resp. Cell Mol. Biol. 28, 339 (2003);
[2] Kim, M. et al. Cell Biochem. Funct. 20, 339 (2002);
[3] Vesey, D. et al. Kidney International 67, 1315 (2005);
[4] Hollenberg, M. Can. J. Physiol. Pharmacol. 75, 832 (1997);
[5] Nishikawa, H. et al. J. Pharmacol. Exp. Ther. 312, 324 (2005). |
| Unit |
Bottle |
| Specification |
1mg 5mg |
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