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Determination of Significance：
Glucose in the blood of mammals is the main form of sugar transport in the body. Blood glucose concentration is regulated by the nervous system and hormones， so it remains relatively stable. While hyperglycemia and hypoglycemia occur when the regulation is out of balance. Hyperglycemia can be caused by diabetes, increased intracranial pressure and dehydration. After the meal, mental tension can also appear physiological high blood glucose. In contrast, hypoglycemia can occur in patients with such conditions as islet cell proliferation or cancer, hypophysis, adrenal cortex and hypothyroidism, and severe liver disease. In addition, hunger and strenuous exercise can cause temporary hypoglycemia.
Measurement Principle：
Glucose oxidase can catalyze the oxidation of glucose to gluconic acid, and produce hydrogen peroxide. Peroxidase catalyzes the oxidation of 4-aminoantipyrine bisphenol by hydrogen peroxide to form colored compounds with characteristic absorption peaks at 505 nm.
Self Provided：
Water-bath, transferpettor, spectrophotometer/microplate reader, micro quartz cuvette/96 well flat-bottom plate and distilled water.
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