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Determination of Significance：
Trehalase(THL, EC 3.2.1.28) is an enzyme found broadly in animals, plants, microorganisms and cultured cells.The main function of THL is to break down trehalose to produce glucose and directly use it for the energy supply of organism.
Measurement Principle：
The 3,5-dinitrosalicylic acid method was used to determine the amount of reducing sugars produced by THL-catalyzed seaweed sugars. The amount of reducing sugar and the depth of color of the reaction solution are proportional to the amount of reducing sugar within a certain range, thus determining the level of THL activity.
Self Provided：
Spectrophotometer/microplate reader, water bath/constant temperature incubator, desk centrifuge, adjustable transferpettor, micro glass cuvette/96 well flat-bottom plate, mortar/homogenizer, ice and distilled water.
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