Determination of Significance:
SDH (EC 1.1.1.14) catalyzes the dehydrogenation of sorbitol to fructose, which is one of the key enzymes to regulate the content of sorbitol in vivo.
Measurement Principle:
SDH catalyzes the dehydrogenation of sorbitol to fructose, and the reduction of NAD+ to NADH. The generated NADH can transfer electrons to NBT to generate purple hairpin. According to this principle, SDH activity can be calculated.
Self Provided:
Spectrophotometer/Microplate reader, desk centrifuge, water bath/ constant temperature incubator, transferpettor, micro glass cuvette/96 well flat-bottom plate, mortar/homogenizer, ice, distilled water.
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