β-glucosidase(β-GC) Activity Assay Kit
Cat.No：BC2565 Solarbio
Detection Method：Spectrophotometer/Microplate Reader
Storage：Store at -20℃，6 months
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Oscillating Mixing & Stirring & Dispersing Instrument
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> Biochemical Assay Kits > Carbohydrate Metabolism Series > Glycometabolic Series > β-glucosidase(β-GC) Activity Assay Kit
Product Information
| Name | β-glucosidase(β-GC) Activity Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at -20℃，6 months |
| Unit | Kit |
| Specification | 100T/48S |
Determination of Significance：
β-glucosidase (β-GC, EC 3.2.1.21) is an enzyme found broadly in animals, plants, microorganisms and cultured cells, which catalyzes the hydrolysis of base-glycosidic bonds and has many physiological functions. In cellulose saccharification, β-GC is responsible for further hydrolysis of cellulose disaccharides and cellulose oligosaccharides to produce glucose. β-GC hydrolyzes the aroma precursors of terpenes, making the glycoside turns from bond state to free state, thus producing the fragrance. β-GC can also hydrolyze wild sakuraside in plants and release HCN, thereby preventing insects from eating.
Measurement Principle：
β-GC can catalyze the p-nitrophenyl-β-D-glucopyranoside to p-nitrophenol. The p-nitrophenol has characteristic of absorption at 400 nm. In this kit, the β-GC activity is quantified by measuring the increase in the color development at 400 nm.
Self Provided：
Spectrophotometer/microplate reader, desk centrifuge, water bath/constant temperature incubator, sonicator, transferpettor, micro glass cuvette/96 well flat-bottom plate, mortar/homogenizer, ice and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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Lysine and threonine supplementation promotes postharvest quality of Volvariella volvacea mushroom during low-temperature preservation via maintenance of the citrate cycle
Click to check >>Author：Ming Gong, Nanyi Li, Tingting Zheng, Jianguo Zhang, Ying Wang, Yongjun Wei, Zhengpeng Li, Erzheng Su, Dapeng Bao, Qi Tan, Gen Zou
IF：6.0560
Publish_to：LWT-FOOD SCIENCE AND TECHNOLOGY
PMID：
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