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Determination of Significance：
α-Galactosidase (α-GAL, EC 3.2.1.22) is an enzyme found broadly in animals, plants, microorganisms and cultured cells. α-GAL catalyze the hydrolysis of α-galactosyl bonds specifically, and mainly participating in the degradation of galactosides such as raffinose, stachyose, melibiose, and galactomannan. α-GAL is crucial for the germination of plant seeds. During the initial stage of seed germination, the D-galactose produced by its catalysis is rapidly transformed and consumed by the glycolytic pathway, which provides the initial source of energy for seed germination. In the later stage, it mainly participates in cell wall storage polysaccharide hydrolysis.
Measurement Principle：
α-GAL can catalyze the p-nitrophenyl-α-pyran galactoside to p-nitrophenol. The p-nitrophenol has characteristic of absorption at 400 nm. In this kit, the α-GAL activity is quantified by measuring the increase in the color development at 400 nm.
Self Provided：
Spectrophotometer/microplate reader, desk centrifuge, water bath/ constant temperature incubator, cell sonicator, transferpettor, micro glass cuvette/96 well flat-bottom plate, mortar/homogenizer, ice and distilled water.
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