Neutral Xylanase（NEX）Activity Assay Kit

Determination of Significance：

Xylanases (EC 3.2.1.8) are mainly produced by microorganisms that catalyse the hydrolysis of xylan, also known as pentosidase or hemicellulase, and are widely used in the brewing and feed industries to break down the cell walls of raw materials and β-glucan in brewing and feed industries, to reduce the viscosity of materials in brewing and facilitate the release of active substances, and to reduce non-starch polysaccharides in feed and facilitate the absorption and utilisation of nutrients. Neutral xylanase (NEX) is generally isolated from microorganisms with an optimum growth pH of 6-8.

Measurement Principle：

NEX catalyzes the degradation of xylan into reducing oligosaccharides and monosaccharides in a neutral environment, and further develops a color reaction with 3,5-dinitrosalicylic acid in a boiling water bath. The color of the reaction solution is proportional to the amount of reducing sugar produced by the enzymatic hydrolysis. The NEX activity can be calculated by measuring the increase rate of the absorbance of the reaction solution at 540 nm.

Self Provided：

Spectrophotometer/ microplate reader, adjustable transferpettor, water bath , mortar/homogenizer, centrifuge, micro glass cuvette/96 well flat-bottom plate ,ice and distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.