Determination of Significance：

Xylanase (EC 3.2.1.8), produced mainly by microorganisms, catalyzes the hydrolysis of xylan, also known as pentosanase or hemicellulase, can break down the cell wall of raw materials in the brewing or feed industry as well as beta-glucan, reduce the viscosity of brewing materials, promote the release of active substances, and reduce non-starch polysaccharides in feed. Promote the absorption and utilization of nutrients, so it is widely used in the brewing and feed industry, acid xylan (ACX) is generally isolated from acid-resistant fungi, bacteria and some molds.

Measurement Principle：

ACX can degrade xylan into reducing oligosaccharides and monosaccharides in acidic environment, and further react with 3, 5-dinitrosalicylic acid in boiling water bath. There is a characteristic absorption peak at 540nm, and the color depth of the reaction solution is proportional to the amount of reducing sugar produced by enzymatic hydrolysis. The activity of ACX can be calculated by measuring the increase rate of absorption value of the reaction solution at 540nm.

Self Provided：

Spectrophotometer/microplate reader, desk centrifuge, water-bath/ constant temperature incubator, adjustable pipette, micro glass cuvette/96 well plate, mortar /homogenizer, ice and distilled water.

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