Determination of Significance：

Xylanase (EC 3.2.1.8) is mainly produced by microorganisms and can catalyze the hydrolysis of xylan, also known as pentosanase or hemicellulase. It can break down the cell walls and β-glucan of raw materials in brewing or feed industries, which reduces the viscosity of materials in brewing, promotes the release of effective substances. As well as it can reduce non starch polysaccharides in feed and promoting the absorption and utilization of nutrients. So it is widely used in brewing and feed industry. Alkaline xylanase (BAX) is generally isolated from microorganisms with an optimal growth pH of 9-11.

Measurement Principle：

BAX catalyzes the degradation of xylan into reducing oligosaccharides and monosaccharides in alkaline environments. Under boiling water bath conditions, it further undergoes a color reaction with 3,5-dinitrosalicylic acid, with a characteristic absorption peak at 540nm. The color depth of the reaction solution is directly proportional to the amount of reducing sugars produced by enzymatic hydrolysis. By measuring the rate of increase in absorbance of the reaction solution at 540nm, BAX activity can be calculated.

Self Provided：

Spectrophotometer/Microplate reader, micro glass cuvette/96 well plate, balance, desk centrifuge, water bath, mortar/homogenizer, adjustable pipette, ice and distilled water.

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