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Determination of Significance：
Cellulase is a multi-component enzyme system produced by microorganisms, which can hydrolyze cellulose β-1,4 glucosidic bonds to produce glucose. Studying the activity of filter paper enzymes is of great significance to the study of cellulase.
Measurement Principle：
The reducing sugar produced by the hydrolysis of the filter paper enzyme can generate red-brown amino compounds with 3,5-dinitrosalicylic acid. It has the maximum light absorption at 540nm. The color of the reaction solution is proportional to the amount of reducing sugar within a certain range. The filter paper enzyme activity can be determined.
Self Provided：
Spectrophotometer/ microplate reader, water bath, balance, centrifuge, adjustable transferpettor, micro glass cuvette/ 96 well flat-bottom plate, mortar/ homogenizer/cell ultrasonic crusher, ice and distilled water, EP tube (2mL).
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
