Glucoamylase Assay Kit

Determination of Significance：

Saccharifying enzyme, glucoamylase (EC3.2.1.3) also known as γ- amylase, which is an extracellular enzyme that is secreted by a series of microorganisms and has exonuclease activity. The main role of Saccharifying enzyme is to hydrolyze α-1,4 glycosidic bonds in sequence from non-reducing ends on the carbon chains such as starch, dextrin, glycogen, etc., and cut off each glucose unit. For amylopectin, when it encounters a branch point, it can also hydrolyze α-1,6 glycosidic bonds, thereby hydrolyzing all amylopectin to glucose. It is one of the important industrial enzyme preparations in China, and is widely used in alcohol, liquor, antibiotics, amino acids, organic acids, glycerol, starch sugar and other industries.

Measurement Principle：

Soluble starch could be hydrolyzed into glucose by saccharifying enzyme. 3, 5-Dinitrosalicylic acid is reduced to brown-red amino compound by co-heating with glucose under alkaline condition. The brown-red amino compound has a maximum absorption peak at 540 nm and the absorbance ratio is in direct proportion to the contents of glucose. The saccharifying enzyme activity is quantified by measuring the color development at 540 nm.

Self Provided：

Microplate reader/spectrophotometer, water bath, balance, desk centrifuge, micro glass cuvette/96 well flat-bottom plate, adjustable transferpettor, mortar/homogenizer/ultrasonic crusher, ice and distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.