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Determination of Significance：
Carbohydrate is one of the important constituents of plants and the main raw materials and storage materials in metabolism. Total sugar mainly refers to reducing glucose, fructose, pentose, lactose and sucrose, maltose, and possibly partially hydrolyzed starch that can be hydrolyzed to reducing monosaccharides under measurement conditions.
Measurement Principle：
The total carbohydrate can be acid hydrolyzed into reduced sugar. In the presence of alkaline solution, the DNS reagent is reduced to an amino compound by co-heating with the reduced sugar, which shows orange-red color and has a maximum absorption peak at 540 nm.
Self Provided：
Spectrophotometer/microplate reader, centrifuge, water bath, transferpettor, micro glass cuvette/96 well flat-bottom plate, mortar/homogenizer, distilled water.
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