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Aniline-4 hydroxylase(AH) Activity Assay Kit

BC2745-241212Manual

Cat.No：BC2745 Solarbio

Detection Method：Spectrophotometer/Microplate Reader

Storage：Store at -20℃，6 months

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Product Information

Name	Aniline-4 hydroxylase(AH) Activity Assay Kit
Detection Method	Spectrophotometer/Microplate Reader
Storage	Store at -20℃，6 months
Unit	Kit
Specification	100T/48S

Determination of Significance：

Cytochrome P450 enzymes are a group of isoenzymes mainly found in the liver. They play an important role in the metabolism of exogenous substances, especially the metabolism of drugs and poisons. Aniline 4-Hydroxylase (AH) is one kind of CYP2E1 isomers in the P450 enzymes family. CYP2E1 not only participate the drug metabolism, but also catalytic the activation of variety of procarcinogens and prepoison.

Measurement Principle：

AH catalyzes the hydroxylation of aniline to produce 4-aminophenol, and further convert to pheno-indole compound, which has a characteristic absorption peak at 630nm. AH activity is calculated by measuring the rate of increase in absorbance at 630nm.

Self Provided：

Spectrophotometer/Microplate reader, constant temperature foster box/water-bath, desk centrifuge, transferpettor, micro glass cuvette/96-well flat-bottom plate, mortar/homogenizer, ice and distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.

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