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Determination of Significance：
Cytochrome P450 enzymes are a group of isoenzymes mainly found in the liver. They play an important role in the metabolism of exogenous substances, especially the metabolism of drugs and poisons. Aniline 4-Hydroxylase (AH) is one kind of CYP2E1 isomers in the P450 enzymes family. CYP2E1 not only participate the drug metabolism, but also catalytic the activation of variety of procarcinogens and prepoison.
Measurement Principle：
AH catalyzes the hydroxylation of aniline to produce 4-aminophenol, and further convert to pheno-indole compound, which has a characteristic absorption peak at 630nm. AH activity is calculated by measuring the rate of increase in absorbance at 630nm.
Self Provided：
Spectrophotometer/Microplate reader, constant temperature foster box/water-bath, desk centrifuge, transferpettor, micro glass cuvette/96-well flat-bottom plate, mortar/homogenizer, ice and distilled water.
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