Determination of Significance:
Potassium maintains normal osmotic pressure and acid-base balance, participates in sugar and protein metabolism, and ensures normal neuromuscular function. If Blood potassium concentration is more than 5.5mmol/L, the disease is called high blood potassium, which increases sensitivity of nerve and muscle, decreases sensitivity of Myocardial stress. When serum potassium concentration exceeds 10mmol/L, ventricular fibrillation or even diastolic arrest occurs. When serum potassium is lower than 3.5mmol/l, hypokalemia can cause muscle weakness, muscle flaccid paralysis, increase of myocardial stress, tachycardia, arrhythmia and even arrest in systolic phase. Therefore, serum potassium is a commonly biochemical index.
Measurement Principle:
Potassium ion in serum is reacted with sodium tetraphenylboron to form potassium tetraphenylboron insoluble in water, and the turbidity is proportional to the concentration of potassium ion in a certain range. The content of serum potassium was determined by measuring its turbidity.
Self Provided:
Centrifuge, Transferpettor, Spectrophotometer/Microplate Reader, Micro Glass Cuvette/96 Well Flat-Bottom Plate, Distilled Water.
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