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Determination of Significance：
Magnesium is the activator of many enzymes, such as phosphatase, creatine kinase, hexokinase and carboxylase. Magnesium is also an essential element for the formation of DNA, RNA and ribosomal macromolecular structures. Meanwhile，magnesium is an important element in maintaining normal nerve and muscle function. Serum magnesium concentration deviating from the normal value is related to some kidney and endocrine diseases, etc.
Measurement Principle：
In alkaline condition, magnesium ions combined with hydroxide ions to colloidal particle, and further turns orange-red color when combined with titan yellow. In a certain range, the absorbance at 540 nm is proportional to the concentration of magnesium ions.
Self Provided：
Transferpettor, spectrophotometer/microplate reader, micro glass cuvette/96 well flat-bottom plate, distilled water.
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