Determination of Significance:
Blood sodium plays an important role in maintaining normal extracellular fluid volume, osmotic pressure and acid-base balance of body fluids.
Measurement Principle:
Sodium and potassium pyroantimonate in serum could precipitate in weak alkaline solution. The amount of precipitate is directly proportional to the concentration of sodium. According to its turbidity, the content of sodium in serum can be determined.
Self Provided:
Spectrophotometer/microplate reader, centrifuge, adjustable pipette, micro glass cuvettes/96-well plate, deionized water and absolute ethanol, 90% ethanol (mix 90mL absolute ethanol and 10mL distilled water).
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