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Determination of Significance：
Hg2+ is an important toxic heavy metal ion in water, which easily absorbed and accumulated by organisms and can be further transmitted through the food chain, causing damage. Minamata disease is a kind of typical mercury poisoning.
Measurement Principle：
After digestion, Hg2+ can form one orange complex with Dithizone in acid environment, which can be dissolved in chloroform and has a maximum absorption peak at 490 nm. In this kit, the content of Hg2+ is quantified by measuring the color development at 490 nm.
Self Provided：
Spectrophotometer/microplate reader, centrifuge, adjusted transferpettor, micro glass cuvette/96 well flat-bottom plate (non-polystyrene/polypropylene material), concentrated sulfuric acid (H2SO4, 98%), concentrated nitric acid (HNO3, 98%), chloroform, distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
