Serum Total Iron Binding Capacity(TIBC) Assay Kit
Cat.No：BC2865 Solarbio
Storage：Store at 2-8℃，6 months
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> Biochemical Assay Kits > Other Biochemical Series > Ion Series > Serum Total Iron Binding Capacity(TIBC) Assay Kit
Product Information
| Name | Serum Total Iron Binding Capacity(TIBC) Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at 2-8℃，6 months |
| Unit | Kit |
| Specification | 100T/96S |
Determination of Significance：
Total iron-binding capacity (TIBC) refers to the ability of serum transferrin to bind iron, and its content is closely related to the diseases such as iron deficiency anemia and acute hepatitis.
Measurement Principle：
Fe2+ reacts with ferrozine to form a fuchsia compound which has an absorption peak at 562nm. In alkaline condition, serum transferrin can bind with Fe3+, and the remaining unbound Fe3+ can be reduced to Fe2+. So the absorbance A1 is positively correlated with Fe3+. After acidification, the transferrin-bound Fe3+ is released and further reduced to Fe2+. The absorbance A2 has a positive correlation with Fe3+, A2 minus A1 was proportional to TIBC.
Self Provided：
Spectrophotometer/microplate reader, water bath/ constant temperature foster box, centrifuge, micro glass cuvette/ 96 well plate, distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Experimental Images
Standard Curve Serum Total Iron Binding Capacity(TIBC) Assay Kit
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