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CAS:11028-71-0
Appearance:White to off-white solid
Storage:Powder : -20℃, 2 years;In solvent(mother liquid): -20℃, 1 month; -80℃, 6 months
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Examples of using this product（for reference only）
In Vitro:
Lymphocyte:
Purified lymphocytes were collected by centrifugation (1800 r,10 min). Lymphocytes were suspended with RPMI 1640 medium containing 4% fetal bovine serum, 2.5 μg/ml ConA (Beijing Solarbio Science Technology Co., Ltd., Beijing, China) and seeded in 6-well plates at a density of 2×106 cells/mL
Reference：
Ma Y, Ye Y, Liu Y, Chen J, Cen Y, Chen W, Yu C, Zeng Q, Zhang A, Yang G. DNMT1-mediated Foxp3 gene promoter hypermethylation involved in immune dysfunction caused by arsenic in human lymphocytes. Toxicol Res (Camb). 2020 Aug 6;9(4):519-529. doi: 10.1093/toxres/tfaa056. PMID: 32905139; PMCID: PMC7467266.
T/B lymphocyte:
The proliferation of spleen T/B lymphocyte was also determined using an MTT assay as above. Briefly, 100 μl of cell suspension was seeded in a well of 96-well culture plates. After the addition of 50 μl Con A (16 μg/mL) or LPS (4 μg/mL) and 50μL COS-Se (80, 200, 400, 800 and 2000 μg/mL), the plates were incubated for 48 h at 37℃ in a 5% humidified CO2. The stimulation index (SI/%) was calculated using the following equation: SI (%) = Absorbance of COS-Se groups/Absorbance of Con A or LPS groups×100%.
Reference：
Jiang Z, Chi J, Li H, Wang Y, Liu W, Han B. Effect of chitosan oligosaccharide-conjugated selenium on improving immune function and blocking gastric cancer growth. Eur J Pharmacol. 2021 Jan 15;891:173673. doi: 10.1016/j.ejphar.2020.173673. Epub 2020 Oct 22. PMID: 33098836.
Lymphocyte :
Within 24 h of the last administration, the mice were sacrificed in a sterile environment. Spleen tissue was disrupted, and spleen cell suspensions were passed through sterile nylon mesh. Red blood cells were lysed by Erythrocyte Lysate (Solarbio, China). Cells were resuspended in RPMI 1640 medium (Solarbio, China) at the concentration of 5 x 106 cells/L. Blastogenic response of splenocytes to the mitogens, concanavalin A (ConA, Solarbio, China), and lipopolysaccharide (LPS, Solarbio, China), were assessed by CCK-8. Splenocytes suspension was incubated with ConA (10 μg/mL) or LPS (15 μg/mL) in 150μL RPMI 1640 medium containing 10% foetal bovine serum at 37 ℃ with 5% CO2. After incubation for 44 h, 10 lL CCK-8 was added to each well. After incubation for 2 h, the absorbance at 450 nm was measured by a microplate reader.
Reference：
Chen M, Fu Q, Song X, Muhammad A, Jia R, Zou Y, Yin L, Li L, He C, Ye G, Lv C, Liang X, Huang J, Cui M, Yin Z. Preparation of resveratrol dry suspension and its immunomodulatory and anti-inflammatory activity in mice. Pharm Biol. 2020 Dec;58(1):8-15. doi: 10.1080/13880209.2019.1699123. PMID: 31847682; PMCID: PMC6968662.
In Vivo:
Mice（Female C57BL/6 mice (6-week-old)，15 mg/kg ConA ，iv. ）:
All the mice were acclimatized for 1 week and then randomized into four groups (n=6-7 per group). According to the previous studies mice were intravenously (i.v.) injected with ConA (15 mg/kg; Solarbio, Beijing, China) for 12 h to establish a mouse model of IMH.
Reference：
Liu M, Zhang H, Zhang L, Liu X, Zhou S, Wang X, Zhong W, Zhang J, Wang B, Zhao J, Zhou L. RIP3 blockade prevents immune-mediated hepatitis through a myeloid-derived suppressor cell dependent mechanism. Int J Biol Sci. 2022 Jan 1;18(1):199-213. doi: 10.7150/ijbs.65402. PMID: 34975327; PMCID: PMC8692153.
