Determination of Significance:
Soil phosphorus includes organic and inorganic phosphorus. the inorganic phosphorous can directly used by plants. Soil organic phosphorus is mineralized and decomposed into inorganic phosphorus. Determine the total phosphorus, organic phosphorus and inorganic phosphorus in the same time can fully reflect the condition of soil phosphorus nutrition.
Measurement Principle:
Molybdenum blue is used to determine phosphorus. One sample of soil is taken and the content of inorganic phosphorus is determined by extraction method. The content of total phosphorus was measured by taking another sample after burning at high temperature. The content of organic phosphorus is calculated by subtracting the content of inorganic phosphorus from the total phosphorus content.
Self Provided:
Spectrophotometer/Microplate reader, micro glass cuvette/96 well flat-bottom plate, centrifuge, water bath, scale, transferpettor, 550 ℃ high temperature electric stove, distilled water and 100 meshes sieve (or smaller).
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